TY  - JOUR
T1  - Antimicrobial Effects of Lysozyme in Combination with <I>Zataria multiflora</I> Boiss. Essential Oil at Different pH and NaCl Concentrations on <I>E. coli</I> O157:H7 and <I>Staphylococcus aureus</I>
AU - , S.S. Saei Dehkordi AU - , S.M. Razavi Rohani AU - , H. Tajik AU - , M. Moradi AU - , J. Aliakbarlou 
JO  - Journal of Animal and Veterinary Advances
VL  - 7
IS  - 11
SP  - 1458
EP  - 1463
PY  - 2008
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2008.1458.1463
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2008.1458.1463
KW  - Early lactation
KW  -milk
KW  -SNF
KW  -CP
KW  -TS
KW  -BF
AB  - The antibacterial efficacy of unheated lysozyme (L) and Heat-Treated Lysozyme (HTL) at 62, 72 and 80°C, separately and in combination with <I>Zataria multiflora</I> Boiss. essential oil (Z.EO) on <I>E. coli </I>O157:H7 and <I>S</I>. <I>aureus</I> at different pH (5, 6 and 7) and NaCl concentrations (0.5, 1.5 and 3%) was studied. A micro-broth dilution assay was applied to define the Minimal Inhibitory Concentrations (MICs). The inhibitory action of L on <I>S. aureus</I> was increased along with pH deduction and NaCl enhancement, whereas <I>E. coli</I> O157:H7 wasn’t inhibited up to 1024 µg mL <SUP>1</SUP> at similar circumstances. Both bacteria were inhibited by HTL (at 72 and 80°C) at NaCl 0.5%, but at  1.5% of NaCl concentration, the inhibition was stopped. However, the inhibitory activity of HTL was greater than L. Z.EO was effective against both bacteria and inhibition was stronger at lower pH and higher NaCl concentrations. MICs of L and HTL combined with Z.EO were decreased. Enhancement of Z.EO concentrations was led to more prominent inhibition, whereas, by increasing NaCl concentration, inhibition  was  reduced considerably. The lowest MICs of L and HTL were achieved by increase of  Z.EO  to 300 µg mL <SUP>1</SUP> along with decrease the pH and NaCl to 5 and 0.5%, respectively.
ER  -