TY  - JOUR
T1  - Determinations of mRNA’s For TGF <SUB> 2, 3 </SUB>in Mouse Testes and Ovaries After a Five days Injection With Glycine or a Serine and Threonine Mixture
AU - , H.B. Ciftci 
JO  - Journal of Animal and Veterinary Advances
VL  - 7
IS  - 1
SP  - 106
EP  - 109
PY  - 2008
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2008.106.109
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2008.106.109
KW  - Mice
KW  -RNA
KW  -TGF
KW  -serine
KW  -threonine
KW  -glycine
AB  - This study is presented to test the effect of injecting a mixture of Serine/Threonine or Glycine amino acids to determine and measure the changes in  mRNAs expression  for TGF <SUB>2, 3 </SUB>within the mouse testes and the ovaries. Male (n = 30) and female  (n  = 30)  mice  (CD-1  strain) were injected, once a day for 5 days, with 0.2 mL Saline (control), 0.2 mL Saline with a mixture of Serine/Threonine (test) containing 0.26 µg L Threonine, 0.13 µg L Serine or 38 ng Glycine (Test). Total RNA was extracted and transferred on to a nylon membrane then probed with <SUP>32</SUP>P-lebelled cDNA probes. The expression of mRNA for TGF <SUB>2</SUB> increased in male mice while there were no differences in female mice. This study shows that TGF <SUB>2, 3 </SUB>are expressed both in the ovary and testis and their expressions were increased by the injection of Serine/Threonine or glycine.
ER  -