TY  - JOUR
T1  - Optimum Ficoll/Hypaque Gradient for Efficient Isolation of Peripheral Blood Monocytes from Camels
AU - , Layla Mohamed AU - , M.M. Mukhtar 
JO  - Journal of Animal and Veterinary Advances
VL  - 6
IS  - 2
SP  - 152
EP  - 154
PY  - 2007
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2007.152.154
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2007.152.154
KW  - Ficoll/Hypaque
KW  -viable cells
KW  -peripheral blood
KW  -PHA
KW  -monocytes
AB  - The viability, Purity and count of monocytes isolated from peripheral blood of camels were assessed after the isolation of these cells on 9, 10 and 12% concentrations of Ficoll/Hypaque. The results obtained showed that 97% viable cells were obtained using the three concentrations of the Ficoll/Hypaque. For purity, 12% Ficoll concentration separated cells were contaminated with others blood cells, while 9 and 10% separated cells were pure. When cells count was applied for the pure isolated cells, the cells isolated on 10% Ficoll/Hypaque concentration showed the best highest   count compared to 9% with p = 0.003. Cells isolated using 10% Ficoll/Hypaque well proliferated when stimulated with Phytohaemaggglutnin (PHA) mitogen.
ER  -