TY  - JOUR
T1  - Alfalfa Ruminal Degradation Using Xylanases
AU - , J.E. Guerra Liera AU - , E. Ibarra Lopez AU - , L.E. Soto Angulo AU - , J.J.R. Hernandez Moreno AU - , J.L. Corrales Aguirre AU - , J. Rodriguez Garcia AU - , L.A. Lopez Juarez AU - , A. Cordova-Izquierdo 
JO  - Journal of Animal and Veterinary Advances
VL  - 6
IS  - 12
SP  - 1443
EP  - 1445
PY  - 2007
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2007.1443.1445
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2007.1443.1445
KW  - Alfalfa degradation
KW  -ruminal digestion
KW  -exogenous enzymes
KW  -xilanases
AB  - Animal production is improved by the use of exogenous enzymes during feeding that modify solubility and digestibility of fiber, which favors a greater ruminal microorganisms activity, this improves profitability of ruminant diets based on grain and forages. There are evidences of the use of these enzymes on diets with a 78% of concentrate, where there was an increase of 25% of NDF ruminal digestion, better than 18% when using 25% of forage. In this research a xilanase enzyme was evaluated Fibrozyme (Alltech, Inc.) which is composed of fermentation extracts of <I>Aspergillus niger</I> and <I>Trichoderma longibrachatium</I>, in four treatments: T1 =  only alfalfa or control (1), T2 =  alfalfa plus Fibrozyme (1), T3 =  alfalfa plus Fibrozyme (2) and T4 =  40% concentrate  plus 60% of alfalfa without Fibrozyme (2), the first two with a basal diet 1 =  100% alfalfa and the last two with 2  =  40% alfalfa plus 60% concentrate for 10 days. The enzyme was given at a dose of 14 g d<SUP>-1</SUP>, 7 g at 0800 and 7 g at 2000. Two male animals ¾ Holstein-Gyr 480 kg average and ruminally cannulated were used. Dry matter offered was adjusted with this equation: 95 g DM (W<SUP>0.75</SUP>). A total of 32 nylon bags 10×20 cm, 50&plusmn;15 micron (Ankom), weighted and identified were introduced in the rumen. Evaluation time was 48 h after bag introduction. Variables evaluated were: ruminal disappearance of Dry Matter, (DM), Neutral Detergent Fiber (NDF) and net energy gain (NEg Mcal kg<SUP>-1</SUP> DM), pH and ruminal temperature. A randomized complete block design, X<SUP>2</SUP> test and Tukey means comparison were performed. Results show that for dry matter, T4 had less residues (30.888<SUP>a</SUP>) (p&lt;0.05) in comparison with the other treatments, which didn&#8217;t show significant differences. The lowest NDF residues after ruminal degradation was in T3 (57.979ª) (p&lt;0.05) with basal diet 2; T1 had the highest values (68.316<SUP>c</SUP>). The greatest net energy gain was on T3, being significantly different to control. Results of comparing treatments including Fibrozyme <I>versus</I> control in relation to NDF 48 h after bag introduction show that when adding Fibrozyme, NDF percentages are in general lower than when Fibrozyme was not added. In the same sense, the highest change percentage (15.13%) was when animals were given alfalfa plus. Fibrozyme with basal diet 2. When comparing means of treatments without Fibrozyme <I>versus</I> the ones with it; superiority of these last was of 10.207 % (p&lt;0.01).
ER  -