TY  - JOUR
T1  - Meiotic Competence of Porcine Oocytes after Percoll Sedimentation Treatment for Oocyte Selection
AU - , Morteza Yavari AU - , Akiko Fujii AU - , Ryohei Shimizu AU - , Aya Ito AU - , Yukine Kaedei AU - , Yasuhiro Morita AU - , Fuminori Tanihara AU - , Takeshige Otoi 
JO  - Journal of Animal and Veterinary Advances
VL  - 6
IS  - 11
SP  - 1333
EP  - 1336
PY  - 2007
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2007.1333.1336
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2007.1333.1336
KW  - Percoll
KW  -sedimentation
KW  -nuclear status
KW  -porcine oocyte
AB  - The objective of this study was to evaluate the effects of Percoll sedimentation treatment of porcine oocytes before <I>In vitro</I> Maturation (IVM) on the meiotic competence of the oocytes. Cumulus-oocyte Complexes (COCs) with uniform ooplasms and compact cumulus cells obtained from porcine ovaries were placed on the surface of Percoll solutions of various concentrations (5, 10, 15, 20 and 25%) in a petri dish. Only the COCs that settled in the dish within 3 min were  transferred into maturation medium. At the end of the IVM culture, the nuclear status of the oocytes was assessed. The proportion of COCs that settled decreased with an increase in the concentration of the Percoll solution. When the COCs were treated with less than 10%  Percoll solution, more than half the total number of COCs settled. In contrast, the proportion of COCs that settled was lower (p< 0.01) in the 20 and 25% Percoll solutions (12 and 1%, respectively) than in Percoll solutions with lower concentrations (49-90%). The proportion of oocytes that underwent germinal vesicle breakdown and reached metaphase II after the Percoll treatment and subsequent IVM culture did not differ among the 5, 10 and 15% Percoll solution groups. Moreover, it also did not differ from that of the control oocytes that were not treated with Percoll. In conclusion, the selection of oocytes by Percoll sedimentation treatment does not improve the rates of nuclear maturation of porcine oocytes and decreases the total number of COCs available for IVM culture.
ER  -