TY  - JOUR
T1  - Simultaneous Identification of Tyrosine Hydroxylase and Choline Acetyltransferase Neurons
AU - , A.O. Ihunwo AU - , R. Schliebs 
JO  - Journal of Animal and Veterinary Advances
VL  - 6
IS  - 9
SP  - 1116
EP  - 1119
PY  - 2007
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2007.1116.1119
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2007.1116.1119
KW  - ChAT
KW  -Tyrosine hydroxylase
KW  -brain
KW  -immunoflurescence
KW  -identification
KW  -neurons
AB  - Tyrosine Hydroxylase (TH) and Choline Acetyltransferase (ChAT) immunoreactivity were examined in the adult mouse brain by a modified double labelling immunofluorescence  method. Mice  were transcardially perfused with saline followed by fixative (4% paraformaldehyde in 0.1M phosphate buffer, pH 7.4). Frozen brain was cut at 30 m thickness for light microscopy. All staining procedures were conducted under room temperature. Dopaminergic neurons were identified from Cy2-coupled-donkey-anti-rabbit antibody and cholinergic neurons by Cy3-coupleddonkey-anti-goat for ChAT. The cell bodies of both TH- and ChAT- positive neurons with their respective axonal and dendritic processes were easily visible. This method proved reliable for simultaneous demonstration, in the same brain tissue sections, of TH- and ChAT-containing neurons.
ER  -