TY  - JOUR
T1  - Cytotoxic Activity in Fermenter Culture Supernatants of<I> Corynebacterium seudotuberculosis</I>
AU - , K.M. Suleima AU - , H. Boehnel AU - , S.H. Babiker AU - , A.Z.S.A. Zaki 
JO  - Journal of Animal and Veterinary Advances
VL  - 5
IS  - 11
SP  - 939
EP  - 942
PY  - 2006
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2006.939.942
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2006.939.942
KW  - Corynebacterium pseudotuberculosis
KW  -fermenter
KW  -cytotoxicity
KW  -cell lines
AB  - Fermenter Culture supernatants of <I>Corynebacterium</I> <I>pseudotuberculosis</I> strain 3450 NCTC grown in a continuous culture were tested for cytotoxicity in 7 cell lines. The supernatants were toxic to EBL, MDCK, NBL, CHO-K1, 3T3, BHK and Vero cells. The cytotoxic activity was used as an assay method to determine the optimal incubation temperature and pH for cytotoxin production in fermenter. Fermenter culture supernatants of a reference strain <I>C. pseudotuberculosis</I> and three field isolates were compared for their cytotoxicity to BHK cells. Two of the field isolates were non-cytotoxic to BHK cells, one isolate had weak cytotoxicity and the reference strain was the most cytotoxic. SDS-PAGE of fermenter culture supernatants of the 4 strains revealed 35 to 39 bands with molecular weights ranging from 24 to 149 KDa.
ER  -