TY  - JOUR
T1  - Preliminary Studies on the Bioactivity of Secondary Metabolites from <I>Aureobasidium pullulans</I> and <I>Emericela rugulosa</I>
AU - , A.K. Onifade 
JO  - Journal of Animal and Veterinary Advances
VL  - 5
IS  - 11
SP  - 930
EP  - 934
PY  - 2006
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2006.930.934
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2006.930.934
KW  - Aureobasidium pullulans
KW  -Emericella rugulosa
KW  -antibacterial
KW  -antifungal
KW  -fungal metabolites
KW  -bioactivity
AB  - In the study of searching for bioactive fungal metabolites, crude extracts of <I>Aureobasidium pullulans </I>and <I>Emericela rugulosa</I> were screened <I>in vitro</I> against eleven human pathogens. The production medium changed from slightly basic (pH 7.21) to slightly acidic (pH 6.72) as fermentation progressed till the 12th day. The wet biomass of the fungal mycelia as well as the dry weights of the crude extracts increased while the amounts of glucose in the growth medium reduced with a corresponding increase in incubation period. Contact bioautographic  assay showed that the extracts exhibited broad-spectrum antimicrobial potency. Activity against  some  human pathogenic organisms including Gram-positive bacteria (<I>Bacillus subtilis</I>, <I>Staphylococcus aureus)</I>,<I>  Gram-negative  bacteria  (Escherichia  coli</I>, <I>  Klebsiella  pneumoniae</I>, <I>Pseudomonas    aeruginosa</I>,<I>    Salmonella    typhi)</I>    and    fungal   strains   (<I>Candida   albicans</I>,<I>   Trichophyton concentrum</I>,<I> T. mentagrophytes</I>,<I> T. rubrum</I> and <I>T. torulans</I>)<I> </I>is discussed<I>.</I> Bioactivity of the extracts, as depicted by zones of inhibition in agar well diffusion assay, was found to increase as the fermentation  days  increased,  irrespective  of  the  fungal  biosource  of  the  metabolites.  In  most  studies, <I>A. pullulans</I> grew faster and caused higher inhibition than <I>E.rugulosa</I>.
ER  -