TY  - JOUR
T1  - Effect of Some Inhibitors on Clostridium Chauvoei (Jakari Strain) Neuraminidase
AU - , N.M., Useh AU - , O.J. Ajanusi AU - , A.J. Nokand K.A.N. Esievo 
JO  - Journal of Animal and Veterinary Advances
VL  - 5
IS  - 9
SP  - 778
EP  - 781
PY  - 2006
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2006.778.781
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2006.778.781
KW  - Inhibitors
KW  -clostridium chauvoei (Jakari strain)
KW  -neuraminidase
AB  - The effect of some inhibitors [silver nitrate (AgNO<SUB>3</SUB>), paranitrophenyl oxamic acid (PNPO), Salicyl Oxamic Acid (SOA) and Ethylene Diamine Tetra Acetic acid (EDTA)] on dialysed Clostridium chauvoei (jakari strain) neuraminidase was investigated <I>in vitro</I> and it was found that these compounds inhibited neuraminidase activity. The concentrations of the inhibitors used were 1.5x10<SUP>3</SUP> µMol, 2.9x10<SUP>3</SUP> µMol, 4.4x10<SUP>3</SUP> µMol, 5.9x10<SUP>3</SUP> µMol and 7.4 µMol for all the inhibitors. Neuraminidase activity was measured by assaying the amount of sialic acid cleaved from fetuin by the enzyme on incubation with the substrate. In all studies, the inhibition was not proportional to the concentration of inhibitor used. It is suggested, based on the present results, that proper clinical trials should be conducted to ascertain the clinical application of these neuraminidase inhibitors in the management of blackleg in cattle.
ER  -