TY  - JOUR
T1  - Detection of New Genomic Landmarks in the Maltese Goat Using Rapd PCR
AU - , Blundell, R. AU - , A.E. Felice 
JO  - Journal of Animal and Veterinary Advances
VL  - 5
IS  - 7
SP  - 602
EP  - 607
PY  - 2006
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2006.602.607
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2006.602.607
KW  - Maltese goat
KW  -DNA
KW  -rapd
KW  -landmark
AB  - Since no information of the Maltese goat genome is available, RAPD technique has been used to identify a number of DNA landmarks. Genome Landmarks have been obtained from the DNA of 66 Maltese goats which were studied with Random Amplification of Polymorphic DNA (RAPD). Eleven (11) reproducible RAPD polymorphic zones were identified. For sequencing, the RAPD zones were cloned into the Puc 18 vector utilising <I>E. coli </I>and then sequenced using both the forward (universal) and reverse primers specific for the Puc 18 vector. After sequencing a total of nine new markers (AF078170, AF078171, AF078172, AF078173, AF078174, AF078175, AF078176, AF078177, AF078178) amounting to approximately 5,500 bp of sequence from the goat genome were identified and their homologies with known nucleic acid and protein databases were described. The new sequences could provide useful anchors for more extensive mapping and sequencing and for genomics assisted breeding of the Maltese goat.
ER  -