TY  - JOUR
T1  - Comparative Polypeptide Profiling: Isolation, Propagation and Purification of Indian Isolates of Buffalopox Virus
AU - , Singh, R.K. AU - , V. Balamurugan AU - , M. Hosamani AU - , C.C. Satheesh AU - , T.J. Rasool AU - , M.P. Yadav 
JO  - Journal of Animal and Veterinary Advances
VL  - 5
IS  - 3
SP  - 260
EP  - 265
PY  - 2006
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2006.260.265
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2006.260.265
KW  - Buffalopox virus
KW  -Indian isolates
KW  -isolation
KW  -propagation
KW  -purification
AB  - Buffalopox is a contagious viral disease-affecting buffaloes (<I>Bubalus bubalis</I>) and rarely cows, with morbidity up to 80%in affected herd causing high economic losses to the farmers. In the present study, as a preliminary work, the field isolates of BPV recovered from suspected clinical materials obtained from outbreaks of different geographical locations of the country were propagated in Vero cells and purified along with reference BP4 virus for comparison. Purification of BPV was carried out in sucrose density gradient (60-36%), where two white opalescent bands appeared after ultracentifugation. The upper band, above the 36% sucrose layer was found to be enveloped virus (Extra cellular enveloped virus) where as the band at interface was the pure  naked  virus  (Intracellular  Mature  Virus).  The  purity  of  the  virus  preparations   was  assessed   by UV-spectrophotometry,  SDS-PAGE  and  infectivity  assay  in  cell   culture.  The  extinction   ratio   values (O.D. 260/280) for all the BPV isolates were within the range of 1.2 to 1.4 indicating highly purified nature of the BPV. On the basis of polypeptide compositions, BPV isolates were similar and contained more than 25 polypeptides with a molecular weight range from 14.2 kDa to 180 kDa. Both the opalescent bands produced characteristics CPE in Vero cell line in infectivity assay. This preliminary study will help in undertaking further work on protein profile and further characterization, which, in turn, will help in understanding the virological and  immunological  properties  of  the  new  virus  isolates  for  development  of  suitable  immuno-diagnostics  and  prophylactics.
ER  -