TY  - JOUR
T1  - Sequencing and Expression of Fimbrial Gene of Pasteurella multocida B:2
AU - , A. Ernie AU - , M. Zamri-Saad AU - , Z. Zunita AU - , A. R. Omar AU - , B. Siti Khairani AU - , M. Y. Sabri 
JO  - Journal of Animal and Veterinary Advances
VL  - 4
IS  - 2
SP  - 254
EP  - 259
PY  - 2005
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2005.254.259
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2005.254.259
KW  - 
AB  - The fimbrial gene of Pasteurella multocida B:2, isolated from cattle with haemorrhagic septicaemia was amplified, cloned, sequenced and expressed in a vector. Cloning of the fimbrial gene revealed a 435 bp band while sequencing revealed similar nucleotide sequencing to the PtfA gene of P. multocida A:3 isolated from chicken, except the five nucleotide changes at residues 171, 282, 363, 387 and 414. The fimbrial gene was found to encode a deduced protein of 144 amino acids. The expressed fimbrial protein was detected from 4 h post-induction with a molecular weight of 18-kDa.
ER  -