TY  - JOUR
T1  - Immunization of Pigs with a DNA Vaccine Against Hog Cholera Virus
AU - , Wu Hongzhuan AU - , Kit-man Wong AU - , Chen Jinding AU - , Frederick C Leung 
JO  - Journal of Animal and Veterinary Advances
VL  - 3
IS  - 4
SP  - 213
EP  - 217
PY  - 2004
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2004.213.217
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2004.213.217
KW  - 
AB  - Two DNA recombinant constructs, pHCV2.5kb and pHCV1.25kb encoding the whole viral structural envelope glycoprotein gene (E2) of Hog Cholera Virus (HCV) were constructed. Those constructs were further delivered by a gene gun or intramuscularly inoculation into pigs respectively. Expression of E2 gene was detected in all vaccinated groups with ELISA, neutralization assay and western blot, but no obvious cellular immune response was observed in T lymphocyte counting test. After challenge with a virulent strain of HCV, it could provide a protection of 57%(16/28).
ER  -