TY  - JOUR
T1  - Polymerase Chain Reaction for the Diagnosis of Human Leptospirosis
AU - , I.M. El Jalii AU - , A. R. Bahaman AU - , A.S. Ali 
JO  - Journal of Animal and Veterinary Advances
VL  - 3
IS  - 4
SP  - 202
EP  - 204
PY  - 2004
DA  - 2001/08/19
SN  - 1680-5593
DO  - javaa.2004.202.204
UR  - https://makhillpublications.co/view-article.php?doi=javaa.2004.202.204
KW  - 
AB  - Polymerase chain reaction (PCR) was evaluated as a tool for diagnosis of human leptospirosis. Human urine samples were seeded with different concentrations of leptospiral cells. Using specific primers, amplified DNA fragments on an agarose gel from urine samples contained as few as 10 hardjo cells per ml urine were obtained. The molecular size of bands produced from all leptospiral concentrations was 600 bp. Urine samples with as little as 10 hardjo cells per ml of urine were positive on PCR indicating high sensitivity of the test. Detection of small number of leptospiral cells in urine by PCR was an advantage over culture and serology.
ER  -