@article{MAKHILLJMG20113128901,
    title = {Optimization the Production of the Sticky Ends of Human <I>CCRL1</I> Gene},
    journal = {Journal of Molecular Genetics},
    volume = {3},
    number = {1},
    pages = {5-7},
    year = {2011},
    issn = {2070-4267},
    doi = {jmolgene.2011.5.7},
    url = {https://makhillpublications.co/view-article.php?issn=2070-4267&doi=jmolgene.2011.5.7},
    author = {Mohammad,Mohammad,Mohadese and},
    keywords = {taq polymerase,T-vector,white colony,blue colony,endonuclease,Iran},
    abstract = {CCRL1 encoded by gene belongs to the family of G-protein coupled receptors. This protein is a kind of C-C chemokine receptors which has a basic role in evolutionary function of heart identified chemokines. The aim of this study is to improve the creation of sticky ends at <I>CCRL1</I> gene by T-vector. In this study, <I>CCRL1</I> gene was amplified by PCR. Restriction sites of BamHI and NotI enzymes were introdused into the 5&prime;end of the forward and reverse primers, respectively. Mentioned enzymes are in the endonuclease family group therefore can not create sticky ends at both sides of <I>CCRL1</I> gene to overcome this problem amplified fragment by Taq polymerase enzyme was inserted into T-vector. Then, recombinant vector was transformed in <I>E. coli</I>.}
    }