@article{MAKHILLVR20158328055,
    title = {The Effect of Various Light Programs on Heterophil to Lymphocyte Ratio and on
Antibodytiter Against Newcastle Disease},
    journal = {Veterinary Research},
    volume = {8},
    number = {3},
    pages = {32-36},
    year = {2015},
    issn = {1993-5412},
    doi = {vr.2015.32.36},
    url = {https://makhillpublications.co/view-article.php?issn=1993-5412&doi=vr.2015.32.36},
    author = {Hadi,Nariman,Alireza and},
    keywords = {Light program,Newcastle antibody titer,Heterophil to lymphocyte ratio,broiler,t-test method},
    abstract = {The light is an important environmental factor
in industrial poultry husbandry. The light programs play
an important role in appropriate management and an
infrastructure for desired function of broilers. In this
study, the effect of various light programs on heterophil
to lymphocyte ratio and on antibody titer against
Newcastle disease with hemaglutination inhibition test
was evaluated. In addition, 800 one-day ROSS 308 broiler
chicken were prepared and randomly divided into four
groups, each group contained 200 chicken, nominated as
A-D. The groups kept at controlled isolated locations,
containing two different light programs. Group A was
adjusted on 23 h lighting and one hour darkness and
group B was designed as recommended program for Ross
breed (2-3 kg end period body weight). Although, group
C and D were considered as control groups and did not
receive Newcastle vaccine. During husbandry period the
groups were in same conditions and just were different in
lighting programs. The groups A and B on 8, 18
and 29 days were vaccinated against Newcastle disease
and on 1, 7, 14, 21, 35, 28 and 42 day old the blood
samples were taken for blood smears for heterophil to
lymphocyte ratio count and antibody titer assessment with
hemaglutination inhibition test. The obtained data were
analyzed with t-test method. The results showed
differences of heterophil to lymphocyte ratio between A
and B groups on 7, 14 and 42 day old which the
ratio was significantly higher in group A than group B
(p<0.05). On 21 and 28 days the significant difference in
this ratio was not observed (p>0.05). On 35 day-old
higher in group B than group A (p<0.05).
Furthermore, the antibody titer of Newcastle vaccine
showed a relative priority in group B on 7, 14, 21,
28, 35 and 42 days than group A whereas no
significant difference were observed in any days (p>0.05).
The results indicated stress condition in group A,
containing lighting program for Ross husbandry
(2-3 kg end body weight) than group B.}
    }