@article{MAKHILLRJANS20071327635,
    title = {Comparative Assessment of Stabilizers Used for Freeze-Drying T<SUB>1</SUB>/44 Contagious Bovine Pleuropneumonia Vaccine (CBPPV) Produce in Nigeria},
    journal = {Research Journal of Animal Sciences},
    volume = {1},
    number = {3},
    pages = {85-88},
    year = {2007},
    issn = {1993-5269},
    doi = {rjnasci.2007.85.88},
    url = {https://makhillpublications.co/view-article.php?issn=1993-5269&doi=rjnasci.2007.85.88},
    author = {E.J. Ngbede},
    keywords = {Stabilizer,freeze-dried,Contagious Bovine Pleuro-Pneumonia Vaccine (CBPPV),assessment of stabilizers},
    abstract = {The effect of using 85% buffered sucrose solution plus gelatin (&quot;S&quot;) as stabilizer and a combination of buffered sucrose/lactalbuminhydrolysate solution in ratio 20:5% (w/w), respectively with gelatin (&quot;L&quot;) as stabilizer during freeze-drying process and under varying storage temperature conditions on CBPP vaccine was investigated. It was found that both stabilizers gave good freeze-dried preparation of T<SUB>1</SUB>/44 CBPP vaccines. During the process of freeze-drying, there was a total average loss of 4.73 cfu mL <SUP>1</SUP>viable organisms in vaccine with stabilizing medium &quot;L&quot; plus gelatin and an average loss of 5.25 cfu mL <SUP>1</SUP> viable organisms for vaccine with stabilizing medium &quot;S&quot; plus gelatin. The results favoured the choice of sucrose/lactalbuminhydrolysate plus gelatin stabilizer on viability of T<SUB>1</SUB>/44 <I>Mycoplasma mycoides </I>subsp. <I>mycoides </I>during freeze-drying process. Vaccine with stabilizing medium &quot;L&quot; plus gelatin stored at <SUP>+</SUP>4°C for 15 months maintained sufficient viable mycoplasma to protect cattle. The vaccine titre dropped from 1.5×10<SUP>9</SUP>-4.3×10<SUP>7</SUP> cfu mL <SUP>1</SUP>, while vaccine with stabilizing medium &quot;S&quot; plus gelatin for the same period dropped from 3.1×10<SUP>9</SUP>-1.8×10<SUP>7</SUP> cfu mL <SUP>1</SUP>. At room temperature of 25°C, the 2 types of vaccines maintained sufficient viable mycoplasma up to 4 months, but detrimental as the titre may drop suddenly when reconstituted for vaccination in the field. Storage of this vaccine at 37°C is not recommended for the same reason stated above.}
    }