@article{MAKHILLJEAS2018132317209,
    title = {Identification of Microbial Community of WAS in Trickling Packed Bed
Reactor Produced Biohydrogen and Studying the Effect of
Hydrolysis by TiO<sub>2</sub> Photocatalysis},
    journal = {Journal of Engineering and Applied Sciences},
    volume = {13},
    number = {23},
    pages = {9935-9945},
    year = {2018},
    issn = {1816-949x},
    doi = {jeasci.2018.9935.9945},
    url = {https://makhillpublications.co/view-article.php?issn=1816-949x&doi=jeasci.2018.9935.9945},
    author = {Ali K.,Mohammad and},
    keywords = {TPBR,COD,pretreatmen,shock rate,microbial,nanoparticles},
    abstract = {In this research, a Trickling Packed Bed Reactor (TPBR) is configured to produce biohydrogen (bio-H<sub>2</sub>) using hydrolysis of an aqueous solution of Waste Active Sludge (WAS) as a substrate in a pilot heterogeneous nanoparticles photocatalysis (UV+TiO<sub>2</sub>). The microbial community in the reactor system was detected which. The results of COD removal% in the photoreactor in TiO<sub>2</sub>+UV, UV and dark after 6 h of operation are equal to 73, 55 and -18.4%, respectively with the presence of spore-forming and gram-negative bacteria. Also, the results show that the acid method was better than the heat one in preparing inoculum for TPBR. And the optimum condition for bio-H<sub>2</sub> production in TPBR is at HRT 8h and pH 5.5 with the presence of microbial community represented by <i>Citrobacter freundii</i>, <i>Bacillus</i> sp., <i>Clostridium</i> sp., <i>Klebsiella oxytoca</i>, <i>Serratia ficaria and Pseudomonas putida</i>. The bio-H<sub>2</sub> volume and COD removal% reach 7.92 mL and 69.55%, respectively in control pretreatment. While <i>Rhizobium radiobacter</i>, <i>Klebsiella pneumoniae</i> sp. <i>pneumoniae</i>, <i>E. coli, Bacillus</i> sp. and <i>Clostridium</i> sp. were indicated in the UV pretreatment that leads to achieving bio-H<sub>2</sub> volume and COD removal % of 22.5 mL and 71%, respectively, the microbial community of <i>Klebsiella pneumonia </i>sp. <i>pneumoniae, Burkholderia mallei, Enterococcus columbae</i>, <i>Bacillus</i> sp., <i>Clostridium</i> sp. and <i>Enterobacter aerogenes</i> were indicated in the TiO<sub>2</sub>+UVpretreatment method with H<sub>2</sub>volume and COD removal% reaching 39.6 mL and 80%, respectively. The maximum bio-H<sub>2 </sub>was produced after supplying organic shock rate, becoming 72 mL after 24 h in the batch state.}
    }