@article{MAKHILLAJ20127320518,
    title = {Effects of Crude Polysaccharides from <I>Catathelasma ventricosum</I> On the Proliferation and Differentiation of 3T3-L1 Cells},
    journal = {Agricultural Journal},
    volume = {7},
    number = {3},
    pages = {187-190},
    year = {2012},
    issn = {1816-9155},
    doi = {aj.2012.187.190},
    url = {https://makhillpublications.co/view-article.php?issn=1816-9155&doi=aj.2012.187.190},
    author = {Xuewei,Xun,Haifeng,Ling,Han,Yuhao,Di,Yuyin and},
    keywords = {Catathelasma ventricosum,polysaccharides,3T3-L1,proliferation,differentation,China},
    abstract = {To study the effects of Crude Polysaccharides from <I>Catathelasma ventricosum</I> (CVCP) on proliferation and differentiation of 3T3-L1 preadipocyte. 3T3-L1 cells were treated with culture media contained different concentrations of CVCP (0, 150, 300 and 600 &#956;g mL<SUP>-1</SUP>) for 24 h and subsequently cell proliferation was assayed by MTT Method. The extent of the differentiation was determined by fluescence spectrophotometry and Nile Red staining on days 8 after treatment with culture media supplemented with different concentration CVCP (0, 150, 300 and 600 &#956;g mL<SUP>-1</SUP>). As the results, the different concentration CVCP (150, 300 and 600 &#956;g mL<SUP>-1</SUP>) promoted proliferation of 3T3-L1 preadipocytes when compared with the control (p&lt;0.05). And it stimulated cell differentiation into adipocyte at low concentration (150 &#956;g mL<SUP>-1</SUP>) while it inhibited cell differentiation into adipocyte at higher concentration (300 and 600 &#956;g mL<SUP>-1</SUP>). But the difference was not significant (p&gt;0.05). Besides, lipid content in 3T3-L1 cells treated with 150 &#956;g mL<SUP>-1</SUP> was significantly higher than treated with 600 &#956;g mL<SUP>-1</SUP> (p&lt;0.05). It can be concluded from the results that the CVCP promoted proliferation of the 3T3-L1 cells 24 h after treatment. Meanwhile, it was involved to some extent in regulation of adipogenesis and lipogenesis.}
    }