@article{MAKHILLIJTM202015119986,
    title = {Entamoeba Typing using Multiplex-PCR and Clinical Features Among Patients with
Inflammatory Diarrhea in Khuzestan, Southwest Iran},
    journal = {International Journal of Tropical Medicine},
    volume = {15},
    number = {1},
    pages = {1-5},
    year = {2020},
    issn = {1816-3319},
    doi = {ijtmed.2020.1.5},
    url = {https://makhillpublications.co/view-article.php?issn=1816-3319&doi=ijtmed.2020.1.5},
    author = {R.,S.,M. and},
    keywords = {PCR,multiplex,histolytica,diarrhea,clinical features,Iran},
    abstract = {Amoebiasis is one of the most important causes
of inflammatory diarrhea. Entamoeba histolytica,
Entamoeba dispar and Entamoeba moshkovskii are
similar morphologically but biochemically and genetically
are different. Therefore, differentiation in Entamoeba
species is one of the most important challenges in
parasitology. In this cross sectional study carried out from
May, 2014 to October, 2015, 242 stool samples that
showed colitis by microscopic examination and then all
specimens evaluated by multiplex-PCR method for the
presence of amoeba and to differentiate the Entamoeba
species. Statistical analysis was performed using SPSS
version 17 and p-<0.05 was considered statistically
significant. Using PCR analysis, of the 242 stool samples,
10 (4.1%) samples were identified as E. histolytica
13 (5.4%) were E. dispar and 2 (0.8%) were E.
moshkovskii. Abdominal pain in 96% of patients
(p<0.001) and nausea and vomiting in 72% of patients
(p<0.001) were the most prevalent clinical manifestations
in patients with amoebiasis. In this study E.dispar was
more prevalent like in other studies. PCR is a gold
standard and highly sensitive and specific molecular
method for differentiated Entamoeba typing to avoid
unnecessary treatment and over diagnosis but since,
PCR is technically expensive and not available
everywhere, we suggest that PCR method use in patients
with abdominal pain and vomiting (The odds ratio of
abdominal pain and vomiting in the amoebiasis group
were 22.7 and 6.8 times more than in the parasite free
group, respectively).}
    }