@article{MAKHILLIJTM20094119765,
    title = {Detection and Differentiation of <I>Echinococcus granulosus-</I>Complex Using A Simple PCR-Based Assay},
    journal = {International Journal of Tropical Medicine},
    volume = {4},
    number = {1},
    pages = {21-26},
    year = {2009},
    issn = {1816-3319},
    doi = {ijtmed.2009.21.26},
    url = {https://makhillpublications.co/view-article.php?issn=1816-3319&doi=ijtmed.2009.21.26},
    author = {Ahmed M.A. Osman,Imadeldin E. Aradaib,Abdel-Latif K. Ashmaig and},
    keywords = {Hydatid disease,cystic echinococcosis,Echinococcus granulosus,complex,genotyping,molecular diagnosis,PCR},
    abstract = {A nested Polymerase Chain Reaction (PCR) assay, for detection of intact and calcified hydatid cysts of <I>Echinococcus granulosus</I> (EG)-complex, was developed and evaluated. The NADH dehydrogenase 1 gene was used as a target DNA for PCR amplification. Two pairs of primers, (EGL 1 and EGR 2) and (EGL 3 and EGR 4), were designed and used in 2 amplification steps. First, the outer pair of primers (EGL 1 and EGR 2), derived from a highly conserve region of NADH 1 gene produced a primary 435 base pair (bp) PCR product from different stains of EG-complex including, sheep strain (genotype) designated (G 1), Cattle strain (G 5), camel strain (G 6) and pig strain (G 7). Second, a pair of internal (nested) primers (EGL 3 and EGR 4), designed internal to the annealing sites of primers (EGL 1 and EGR 2), produced a 276 bp PCR product. The primary 435 bp and the nested 276 bp EG specific PCR products were easily identified following visualization onto an ethidium bromide-stained agarose gel. However, the primary or the nested PCR products were not amplified from DNA extracted from <I>Cysticercus tenuicollis</I> and <I>Coenurus cerebralis</I>, the larval stages of the adult dog cestodes <I>Taenia hydatigena</I> and <I>T. multiceps</I>, respectively. The described nested PCR assay could be used to detect sheep, cattle and camel strains of EG-complex circulating in Sudan. This PCR assay could also, be used for rapid detection and differentiation of EG-complex from other related cestodes. This assay should be considered during an epidemiological survey of the disease in areas of endemicity.}
    }