@article{MAKHILLIJTM20061219679,
    title = {Comparison of Coventional Isolation, Phage-Based Assay and PCR for Detection of <I>Mycobacterium tuberculosis </I>Complex},
    journal = {International Journal of Tropical Medicine},
    volume = {1},
    number = {2},
    pages = {48-52},
    year = {2006},
    issn = {1816-3319},
    doi = {ijtmed.2006.48.52},
    url = {https://makhillpublications.co/view-article.php?issn=1816-3319&doi=ijtmed.2006.48.52},
    author = {Haitham A. Albir,Suliman M. ElSanousi,Tarig G. Eldawi,Mohamed E. Ahmed and},
    keywords = {Conventional isolation,mycobacterium tuberculosis,PCR},
    abstract = {In the present study, conventional bacterial isolation, bacteriophage-based assay (FAST Plaque TB)
and Polymerase Chain Reaction (PCR) were evaluated for detection of <I>Mycobacterium tuberculosis </I>complex.
A total of 47 mycobacterial isolates consisting of 38 isolates of <I>Mycobacterium tuberculosis </I>complex and 9
isolates of mycobacteria other than <I>M. tuberculosis </I>complex were used in this study. In addition, nine reference
strains of Mycobacterium consisting of 7 <I>Mycobacterium tuberculosis, </I>one strain <I>of Mycobacterium
flavescen</I>s, one srtrain of <I>mycobacterium duvalii </I>were also used in this study. Conventional isolation is
laborious, cumbersome and time consuming where it takes as long as 2 months for definitive diagnosis. The
phage assay is sensitive and it takes only two working days. PCR is a rapid assay and definitive diagnosis of
tuberculosis infection could be made possible within the same working day. The described
bacteriophage-based assay could be used as rapid, sensitiveand specific method to support the currently
available conventional methods used for detection of <I>Mycobacterium tuberculosis </I>in developing countries.}
    }