@article{MAKHILLRJP20093112422,
    title = {<I>In vitro</I> Response of Cancer Cells to the Growth-Inhibitory Effects of Dichloromethane Extract of <I>Goniothalamus umbrosus</I>},
    journal = {Research Journal of Pharmacology},
    volume = {3},
    number = {1},
    pages = {1-6},
    year = {2009},
    issn = {1815-9362},
    doi = {rjpharm.2009.1.6},
    url = {https://makhillpublications.co/view-article.php?issn=1815-9362&doi=rjpharm.2009.1.6},
    author = {Ahmad Bustamam Abdul,Siddig Ibrahim Abdel-Wahab,Hew Kim Fong,Syam Morali Mohan,Adel Sharaf Al-Zubairi and},
    keywords = {Goniothalamus umbrosus,breast cancer,apoptosis,MCF-7,HT-29},
    abstract = {This present study aims to investigate whether <I>Goniothalamus umbrosus</I> extract has anticancer effects on several human cancer cells namely, the MCF-7 breast cancer cell line, HT-29 colon cancer cell line and CEM-ss leukemia cell line. Morphological changes and probable mode of cancer cell death induced by these <I>G. umbrosus</I> extract was examined. The dichloromethane plant extract of <I>G. umbrosus</I> was investigated for its cytotoxic activities on the human cancer cells using a 3 days MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay. The MTT assay results revealed that the extract has anticancer effects on human breast cancer cells (MCF-7). Half maximal inhibitory concentration (IC<SUB>50</SUB>) of the hexane extract was found to be 19.5&plusmn;0.338 mg mL<SUP>-1</SUP> and this concentration was further used in investigations towards MCF-7 human breast cancer cells. Both inverted and fluorescence microscopic studies demonstrated that treated MCF-7 breast cancer cells using IC<SUB>50</SUB> of the extract displayed a number of typical morphological changes. Appearance of membrane blebs, DNA condensation and fragmentation are significant signs of apoptosis. The above findings suggested, that the hexane extract of <I>Goniothalamus umbrosus</I> has potential therapeutic effect towards human breast cancer cells that requires further investigations in future.}
    }