@article{MAKHILLRJMS20082411630,
    title = {<I>In vitro</I> Antileishmaniasis, Phyto and Cytotoxicity of <I>Pycnanthus angolensis</I> Methanolic Extracts},
    journal = {Research Journal of Medical Sciences},
    volume = {2},
    number = {4},
    pages = {178-181},
    year = {2008},
    issn = {1815-9346},
    doi = {rjmsci.2008.178.181},
    url = {https://makhillpublications.co/view-article.php?issn=1815-9346&doi=rjmsci.2008.178.181},
    author = {P.A. Onocha,E.O. Ajaiyeoba and},
    keywords = {Pycnanthus angolensis,Myristicaceae,methanolic extracts,antileishmanial,phytotoxicty,cytotoxicity},
    abstract = {Antileishmaniasis, phytotoxicity and cytotoxicity of the methanolic extracts of the root, stem and leaves of <I>Pycnanthus angolensis </I>was evaluated <I>in vitro</I>, as part of the screening of ethno - medically useful plants from the Nigerian flora for biological activity and constituents. Brine shrimp lethality has been extensively used as a  tool  to  screen  active  natural  products.  Bioactive compounds are often toxic to <I>Artemia salina</I> (shrimp eggs) and it has been observed that natural antitumor compounds can inhibit the growth of<I> Lemna minor</I>. The antileishmanial activity was assessed using promastigote culture of Pakistani leishmanial strain (<I>L. major</I>) in 96 well micro titer plate bioassay, phytotoxicity using the <I>Lemna</I> bioassay and cytotoxicity using brine shrimp lethality assay. The methanolic extract of the root and ste<I>m</I> exhibited dose dependent phytotoxicity while the leaf methanolic extract only displayed significantly phytotoxicity at the highest  dose investigated.  The  stem  methanolic  extract  was  found  to  be  leishmanicidal with an IC<SUB>50</SUB> of 70.59<I> </I>µg mL<SUP> 1</SUP>and exhibited no cytotoxicity. The root methanolic extract exhibited cytotoxicity with a positive lethality of LD<SUB>50</SUB> 727.70 µg mL<SUP> 1</SUP> and was not leishmanicidal. The leaf methanolic extract was neither leishmanicidal nor cytotoxic. These results could be considered a valuable support of the ethnomedical uses of the plant.}
    }