@article{MAKHILLRJMS20082411628,
    title = {Analysis of Variability of Clones and Subclones of <I>Trypanosoma cruzi </I>Derived from Mexican Strains by the Behavior in Mice and Culture Cells},
    journal = {Research Journal of Medical Sciences},
    volume = {2},
    number = {4},
    pages = {166-174},
    year = {2008},
    issn = {1815-9346},
    doi = {rjmsci.2008.166.174},
    url = {https://makhillpublications.co/view-article.php?issn=1815-9346&doi=rjmsci.2008.166.174},
    author = {Marco Antonio Becerril Flores,Salazar Schettino Paz Maria and},
    keywords = {Variability,clones,T. cruzi,mice and culture cells},
    abstract = {To better understand the biological heterogeneity of behavior of <I>Trypanosoma cruzi</I> strains isolated from Mexico, 6 strains of <I>T. cruzi </I>were cloned by the Miles’ method (drops of suspension with parasites were diluted in phosphate-buffered saline solution and inoculated to mice).Virulence and infectivity of 10 or 11 clones derived from each strain were determined in female Balb/c mice and Vero culture cells, respectively. Variability of clones was determined by Tukey F statistic test. Only 1 strain, T5, showed interclonal variability and its clones were subcloned by the same method; they showed similar behavior as their parental clones. Clones increased the virulence or had the same behavior after maintaining them for 1 year in mice. Seven clones were eliminated and the virulence of the 4 remaining clones was attenuated when they were maintained in LIT axenic culture for 1 year. The study showed that the strains of <I>T. cruzi</I> in Mexico are either monoclonal or polyclonal and the clones could be eliminated or selected from the environment along time; therefore it is possible to observe that the behavior of <I>T. cruzi</I> strains can change when they are maintained in laboratory for several years.}
    }