@article{MAKHILLRJAS2011639110,
    title = {Assessment of Sperm Viability, Determination Fertilization Capacity and Hatching Rate by Artificial Insemination of Banana Shrimp, <I>Penaeus merguiensis</I> (De Man, 1888)},
    journal = {Research Journal of Applied Sciences},
    volume = {6},
    number = {3},
    pages = {174-178},
    year = {2011},
    issn = {1815-932x},
    doi = {rjasci.2011.174.178},
    url = {https://makhillpublications.co/view-article.php?issn=1815-932x&doi=rjasci.2011.174.178},
    author = {A.J.,M.,A.D.,M.I.,M.O.,J. and},
    keywords = {Banana shrimp,Penaeus merguiensis sperm viability,artificial insemination,fertilization,spermatophore,shrimps},
    abstract = {The study was conducted to every hour assess the sperm viability of banana shrimp (<I>Penaeus merguiensis</I>) collected from Kedah water, West Malaysia (5&deg;39'N; 100&deg;19'E). Evaluations were done on three ways: group A: whole fresh specimens maintained at 2&deg;C prior to extraction, group B: whole spermatophore maintained at 2&deg;C and C: sperm suspension maintained at 2&deg;C. Spermatophores counts were determined by sperm suspension using modified eosin-nigrosin staining method. Percentage of mean sperm viability for the fresh sample in group A at time zero was 93.96&plusmn;3.74%, B was 98.8&plusmn;0.7% and C was 99.02&plusmn;0.7%. In group A, viability of the sperm considerably decreased after the first 60 min was 76.4&plusmn;5.96% but in group B and C was gradually decreased at 91.4&plusmn;0.9 and 97.6&plusmn;1.2%. These were 67.9&plusmn;5.13 and 62.5&plusmn;5.1% in group A, 82.3&plusmn;1.4% and 75.9&plusmn;2.2% in group B and in group C was 93.3&plusmn;1.9 and 88.2&plusmn;3.1%, respectively after 1st-2nd h times elapsed. At 7th h in all groups the viability decreased significantly to &lt;60% (p&lt;0.05). Following, mean sperm viability were considerably decreased to group A was 50.7&plusmn;4.79 and 37&plusmn;6.52%, group B was 51.2&plusmn;1.6 and 31.6&plusmn;1.4% and group C was 56.2&plusmn;1.9 and 41.5&plusmn;2.3%, respectively after 7th and 9th h. However, for spermatophores in group A after 7th h fertilization and hatching rate was 44.3 and 64.4% in group B was 61.9 and 67.7% and group C was 42.9 and 61.3%, respectively at rates comparable to control 88.2 and 76.2%, respectively. There was no significant relationship was observed between biomass of the spermatophore to the body weight of the shrimp (p&gt;0.05). The present study also revealed that specimens, spermatophore or sperm suspension maintained at 2&deg;C could be utilized for fishery management through artificial insemination process till 7th h.}
    }