@article{MAKHILLRJAS2010569066,
    title = {Biohydrogen Production by Anaerobic Biofilms from a Pretreated Mixed Microflora},
    journal = {Research Journal of Applied Sciences},
    volume = {5},
    number = {6},
    pages = {376-382},
    year = {2010},
    issn = {1815-932x},
    doi = {rjasci.2010.376.382},
    url = {https://makhillpublications.co/view-article.php?issn=1815-932x&doi=rjasci.2010.376.382},
    author = {Jesus,Jose A.,Yolanda,Jose G.,Leopoldo J. and},
    keywords = {dairy wastewater,Opuntia imbricata,metagenomic,hydrogen,Fuel,firmicutes},
    abstract = {The present research focused on fermentative production of hydrogen by anaerobic biofilms from a pretreated mixed microflora in a continuous fixed bed-reactor. Microbial mixed culture was thermally (100&deg;C, by 30 min) and acidic (pH 3.0, 24 h) pretreated. Metagenomic analysis of the pretreated microbial cultured was conducted. Microbial cultured community was mainly formed by lactic acid bacillus from <I>Phylum firmicutes</I>. Dairy wastewater (COD 27.73 g L<SUP>-1</SUP>, pH 11.3) at a temperature of 18&plusmn;5&deg;C was fed to a 1.5 L continuous fixed bed-reactor using <I>Opuntia imbricata </I>as substratum. Results obtained showed that at an HRT of 0.4 h the yield hydrogen production was 2.11 mmol H<SUB>2 </SUB>g<SUP>-1</SUP> COD<SUB>consumed</SUB>, mean while an increase in HRT to 0.8 h caused a decrease in yield hydrogen production to 1.31 mmol H<SUB>2 </SUB>g<SUP>-1</SUP> COD<SUB>consumed</SUB>.}
    }