@article{MAKHILLRJAS2009428978,
    title = {<I>In silico</I> Annotation of the Genes Involved in Biosynthesis of Lipopolysaccharide for <I>Burkholderia pseudomallei</I>},
    journal = {Research Journal of Applied Sciences},
    volume = {4},
    number = {2},
    pages = {62-72},
    year = {2009},
    issn = {1815-932x},
    doi = {rjasci.2009.62.72},
    url = {https://makhillpublications.co/view-article.php?issn=1815-932x&doi=rjasci.2009.62.72},
    author = {Nagi A.,Lai L.,Zamberi,Mariana N.,Rasedee and},
    keywords = {Melioidosis,Burkholderia pseudomallei,open reading frames,lipopolysaccharides,bioinformatics,data mining,genes},
    abstract = {<I>Burkholderia pseudomallei</I> is the causative agent of melioidosis, a serious disease of man and animals. The high mortality of <I>B. pseudomallei</I> infections may cause by lipopolysaccharides, an endotoxin. The biosynthesis of LPS is complex comprising three components, lipid A, core oligosaccharide and O-specific antigen. In the current study, by using the available <I>B. pseudomallei</I> genome database provided by Wellcome. The study demonstrated that the bioinformatics comparative technique was able to annotate LPS genes in <I>Burkholderia pseudomallei</I>. By developing a simple and easy flow chart including the using of Artemis software, total of 44 putative ORFs involved in biosynthesis of lipopolysaccharide for <I>B. pseudomallei</I> and the genetic mapping for the ORFs have been successfully determined using bioinformatics and laboratory approach. It is about 95.7% of success for annotation based on the 46 genes that act as references. In near future, a suitable vaccine or antimicrobial may be developed by targeting the genes encoding the various components essential in LPS biosynthesis and survival of the pathogen.}
    }