@article{MAKHILLRJBS20138211395,
    title = {Influence of Physical and Chemical Parameters on Protease Production by <I>Bacillus megaterium</I> IBRL MS 8.2 in Submerged Fermentation},
    journal = {Research Journal of Biological Sciences},
    volume = {8},
    number = {2},
    pages = {54-61},
    year = {2013},
    issn = {1815-8846},
    doi = {rjbsci.2013.54.61},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2013.54.61},
    author = {S.H.,I. and},
    keywords = {Bacillus megaterium,submerged fermentation,proteases,enzyme production,Malaysia},
    abstract = {Proteases are a group of enzymes that catalyze the hydrolysis of protein and among the various proteases, bacterial proteases are the most significant when compared to animal and fungal proteases. In this study, a local bacterial isolate <I>Bacillus megaterium</I> IBRL MS 8.2 was found to produce the highest protease production of 155.38 U mL<SUP>-1</SUP> when cultivating it in the improved medium which consisted of initial medium pH of 7.5, inoculum size of 7.5% (3x10<SUP>7</SUP> cells mL<SUP>-1</SUP>), agitation speed of 150 rpm, incubation temperature of 30&deg;C, 0.5% of sucrose and 1.0% of beef extract. The highest protease production was achieved on the 30 h of cultivation. On the other hand, the highest cell growth of 2.33 g L<SUP>-1</SUP> was achieved on 18 h of cultivation time. The data obtained suggest that enzyme production by <I>B. megaterium</I> IBRL MS 8.2 was not growth dependant.}
    }