@article{MAKHILLRJBS20138611422,
    title = {Profile of VEGF Secretion in Human Peripheral Blood Mononuclear Cells <I>In vitro</I>},
    journal = {Research Journal of Biological Sciences},
    volume = {8},
    number = {6},
    pages = {210-214},
    year = {2013},
    issn = {1815-8846},
    doi = {rjbsci.2013.210.214},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2013.210.214},
    author = {Fatemeh},
    keywords = {VEGF,profile,human,PBMCS,therapeutic},
    abstract = {Peripheral Blood Mononuclear Cells (PBMCs) are key regulators of inflammation. Vascular Endothelial Growth Factor (VEGF) is a cytokine which is produced by a number of cells and has an important role in inflammation. In this study, the patterns of VEGF expression by human PBMCs were assessed <I>in vitro</I> using Phytoheamagglutinin (PHA), Phorbol Myristate Acetate (PMA) and Lypopolysaccharide (LPS) as VEGF inducers. Human PBMCs were cultured in complete RPMI-1640 medium. Then, the cells were seeded at a density of 10<SUP>6</SUP> cells mL<SUP>-1</SUP> and subsequently were incubated with PMA (0-25 ng mL<SUP>-1</SUP>), PHA (0-10 &#956;g mL<SUP>-1</SUP>) or LPS (0-4 &#956;g mL<SUP>-1</SUP>) for 48 h. The level of VEGF secreted in the cell culture supernatants was measured with enzyme-linked immunosorbent assay kits (R and D systems). Human PBMCs cultured without any stimulus, produced detectable amount of VEGF after 48 h incubation. PMA significantly reduced while PHA/LPS significantly increased VEGF production in human PBMCs dose-dependently. The results show that PMA, PHA and LPS have different effects on VEGF production by PBMCs.}
    }