@article{MAKHILLRJBS20138511407,
    title = {Evaluation of Cleaning Protocols on Endodontic Instruments},
    journal = {Research Journal of Biological Sciences},
    volume = {8},
    number = {5},
    pages = {124-128},
    year = {2013},
    issn = {1815-8846},
    doi = {rjbsci.2013.124.128},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2013.124.128},
    author = {Amin,Mohammad G.,Morteza and},
    keywords = {Cleaning protocol,endodontic instruments,biological debris,sponge,chemical},
    abstract = {To evaluate an effective cleaning protocol for endodontic 
  instruments. Total 180 new endodontic k-files (sizes 15, 25 and 40) were contaminated 
  by preparing canals of extracted teeth. The practices used different cleaning 
  protocols for the instruments. These protocols involved four components the 
  use of sponges soaked with chlorhexidine, brushing, pre-soaking in MICRO 10 
  ENZYME and ultrasonication. After cleaning, the files were immersed in Van Gieson’s 
  solution and examined under magnification for stained debris. The effectiveness 
  of cleaning sequences was tested on the instruments. The mean percentage of 
  highest contamination score was 35% in sponge soaked with chlorhexidine gluconate 
  group, 15% in the brushing only group, 5% in MICRO 10 ENZYME soak plus brushing 
  group, 0% in MICRO 10 ENZYME soak and brushing and ultrasonication group and 
  4% in ultrasonicatin only group. There were no clean instruments in these groups 
  except group 4. There was a statistically significant difference between the 
  full cleaning method (group 4) and other groups (p<0.05). Statistically, 
  size of instruments did not significance influence on debris removal. The best 
  method for the efficient debris removal was the one that included mechanical, 
  chemical and ultrasonic cleaning of instruments.}
    }