@article{MAKHILLRJBS20127111321,
    title = {Development of Cultivation Media for Polyhydroxyalkanoates Accumulation in Bacterial Cells Isolated from Cassava Pulp},
    journal = {Research Journal of Biological Sciences},
    volume = {7},
    number = {1},
    pages = {31-37},
    year = {2012},
    issn = {1815-8846},
    doi = {rjbsci.2012.31.37},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2012.31.37},
    author = {Onuma,Hathairat and},
    keywords = {Development,cultivation media,polyhydroxyalkanoates,accumulation,E. coli,Thailand},
    abstract = {Polyhydroxyalkanoates (PHAs) are biopolymers efficiently used as biodegradable plastics to replace environmentally unfriendly petroleum-derived plastics. The polymers can be synthesized by a wide range of microorganisms. Bacteria accumulate PHAs under conditions of nutrient stress particularly nitrogen or phosphorus limitations. In this study, cultivation media were developed for detecting a number of bacteria isolated from cassava pulp for their PHA production capability by modifying media described by potential references. Both complex and minimal media were developed when cultured at 35&deg;C for 48 h. The isolates were tested for the presence of PHA accumulation by straining with 1% Nile blue A and observed under the fluorescence microscope at excitation wave length of 650 nm. Transmission Electron Microscope (TEM) was used for comfirmation of PHA granules accumulation in bacterial cells. <I>Alcaligenes eutrophus </I>(TISTR 1095) and <I>E. coli </I>(TISTR 527) were used as the positive and negative control of PHAs-producing strain, respectively. This is the first report for the suitable media for detecting of PHAs-producing bacteria isolated from cassava pulp.}
    }