@article{MAKHILLRJBS20116111175,
    title = {Phylogenetic Analysis of Lysozyme C from the Scorpion <I>Mesobuthus eupeus</I> Venom Gland},
    journal = {Research Journal of Biological Sciences},
    volume = {6},
    number = {1},
    pages = {9-11},
    year = {2011},
    issn = {1815-8846},
    doi = {rjbsci.2011.9.11},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2011.9.11},
    author = {Ghafar,Nabiallah and},
    keywords = {amino acids,anti-microbial protein,Iran,scorpion venom,Phylogenetic analysis,lysozyme C},
    abstract = {Many studies have been carried out on peptides and genes encoding scorpion toxins from the venom of the scorpion <I>Mesobuthus eupeus</I>. The scorpion venom contains a diversity of bioactive peptides which could cause toxic effects and can be candidates for drug design and development. The anti-microbial lysozymes among them are of great value. Lysozymes are hydrolytic enzymes characterized by the ability to cleave the &beta;-(1, 4)-glycosidic bond between N-acetylmuramic acid and N-acetyl-D-glucosamine in a peptidoglycan layer, the major bacterial cell wall polymer. The total RNA was extracted from venom glands of <I>Mesobuthus eupeus</I> species of Kuzestan. cDNA was synthesized with extracted total RNA as template and modified oligo (dT) as primer. In order to amplify cDNA encoding a Lys-C peptide, semi-nested RT-PCR was performed with the specific primers followed by sequencing of the amplified fragment. The full-length cDNA sequence contains a 438 nucleotide open reading frame encoding a peptide of 144 amino acids with molecular weight of 16.702 kDa. A putative 22-residue signal peptide was identified. Based on the phylogenetic tree of MesoLys-C and c-type lysozyme of East Mediterranean <I>M. eupeus</I> it is concluded that <I>M. eupeus</I> of Khuzestan and East Mediterranean <I>M. eupeus</I> belong to different subspecies.}
    }