@article{MAKHILLRJBS201161111298,
    title = {Effects of the Crude Polysaccharides from <I>Poria cocos</I> on the Proliferation and Differentiation of 3T3-L1 Cells},
    journal = {Research Journal of Biological Sciences},
    volume = {6},
    number = {11},
    pages = {597-601},
    year = {2011},
    issn = {1815-8846},
    doi = {rjbsci.2011.597.601},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2011.597.601},
    author = {Xun,Haifeng,Ling,Han,Chunyou,Xuewei and},
    keywords = {Poria cocos,polysaccharides,3T3-L1,proliferation,differentiation,China},
    abstract = {To study the effects of crude polysaccharides from <I>Poria cocos</I> (PCCP) on proliferation and differentiation of 3T3-L1 preadipocyte. 3T3-L1 cells were treated with culture medium contained different concentrations of PCCP (0, 50, 100 and 200 &#956;g mL<SUP>-1</SUP>) for 48 h and subsequently cell proliferation was assayed by MTT Method. The extent of the differentiation was determined by fluescence spectrophotometry and Oil Red O staining on days 8 after treatment with culture medium supplemented with different concentration PCCP (0, 50, 100 and 200 &#956;g mL<SUP>-1</SUP>). As the results, the different concentration PCCP (50, 100 and 200 &#956;g mL<SUP>-1</SUP>) inhibited to some extent proliferation of 3T3-L1 preadipocytes when compared with the control. But the difference is not significant (p&gt;0.05). And PCCP stimulated cell differentiation into adipocyte at lower concentration (50 and 100 &#956;g mL<SUP>-1</SUP>) (p&lt;0.05) while it inhibited cell differentiation into adipocyte at high concentration (200 &#956;g mL<SUP>-1</SUP>) (p&gt;0.05). It can be concluded from the results that the PCCP was involved in regulation of adipogenesis and lipogenesis.}
    }