@article{MAKHILLRJBS20116211186,
    title = {Cytotoxic Activity of <I>Lythrum salicaria </I>L.},
    journal = {Research Journal of Biological Sciences},
    volume = {6},
    number = {2},
    pages = {55-57},
    year = {2011},
    issn = {1815-8846},
    doi = {rjbsci.2011.55.57},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2011.55.57},
    author = {Mahnaz,Mana,Azadeh,Mohamad,Mahdi,Yousef and},
    keywords = {Cytotoxic activity,Lythrum salicaria L.,MTT assay,fraction,chloroform,cell,Iran},
    abstract = {The aerial parts of <I>Lythrum salicaria </I>L. from Lahijan in Gillan province have been collected in June 2009. Total extract were obtained by MeOH/H<SUB>2</SUB>O (80/20) and then partitioned between CHCl<SUB>3</SUB>, EtOAc and MeOH. These fractions and total extract have been investigated for in vitro cytotoxic activity against colon carcinoma (HT-29), lukemia (K562), breast ductal carcinoma (T47D) and Swiss mouse embryo fibroblast (NIH 3T3) cell lines by MTT assay (3-(4,5-di methyl thiazol-2-yl)-2,5-di phenyltetrazolium bromide). At each cell line doses of 50, 100, 200, 400 and 800 &#956;g mL<SUP>-1</SUP> in 1% (v/v) DMSO of all samples were tested. Ethyl acetate fraction against proliferation of T47D cell line has been represented high cytotoxic activity (IC<SUB>50</SUB>&lt;70). Chloroform fraction and total extract against proliferation of T47D cell line and chloroform fraction toward K562 cell line and total extract against HT-29 cell line have been demonstrated slightly cytotoxic activity (IC<SUB>50</SUB>&lt;200). The other fractions have been demonstrated no effective cytotoxic activity. IC<SUB>50</SUB> values confirmed that the growth and proliferation of T47D cells were most affected by ethyl acetate fraction.}
    }