@article{MAKHILLRJBS20116511237,
    title = {Growth Characteristics and Halocin Production by a New Isolate, <I>Haloferax volcanii </I>KPS1 from Kovalam Solar Saltern (India)},
    journal = {Research Journal of Biological Sciences},
    volume = {6},
    number = {5},
    pages = {257-262},
    year = {2011},
    issn = {1815-8846},
    doi = {rjbsci.2011.257.262},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2011.257.262},
    author = {P.,A.P.,A.R. and},
    keywords = {Haloferax volcanii KPS1,halocin KPS1,halophilic agar,culture conditions,enzymes,India},
    abstract = {An archeon <I>Haloferax volcanii</I> KPS1 was isolated from Kovalam saltern, Kanyakumari, India. It produced halocin which was active against gram positive and gram negative bacteria viz., <I>Bacillus subtilis</I>,<I> Streptococcus mutans</I>,<I> Staphylococcus aureus</I>,<I> Escherichia coli</I> and <I>Pseudomonas aeruginosa</I>, respectively. The halobacteria <I>H. sodomens </I>was inhibited to the maximum by the halocin KPS1 of the <I>Haloferax volcanii</I> KPS1. The growth and halocin production was maximized when the organism was grown at a temperature of 40&deg;C at the pH of 7.0 and when supplemented with 25% w/v NaCl to the halophilic broth medium. The results of the stability studies indicated that the halocin KPS1 became thermolabile at the temperature &gt;80&deg;C and was stable over a wide range of pH from 3.0-9.0. A loss of activity of halocin KPS1 was detected when treated with proteolytic enzymes like proteinase K and trypsin indicating the proteinaceous nature of the antibacterial compound. The halocin produced by <I>Haloferax volcanii</I> KPS1 would provide a lead for developing antimicrobial drugs.}
    }