@article{MAKHILLRJBS20094210837,
    title = {Oncolysis of Breast, Liver and Leukemia Cancer Cells Using Ethyl Acetate and Methanol Extracts of <I>Goniothalamus umbrosus</I>},
    journal = {Research Journal of Biological Sciences},
    volume = {4},
    number = {2},
    pages = {209-215},
    year = {2009},
    issn = {1815-8846},
    doi = {rjbsci.2009.209.215},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2009.209.215},
    author = {Siddig Ibrahim Abdel-Wahab,Ahmad Bustamam Abdul,Syam Murali Mohan,Adel Sharaf Al-Zubairi and},
    keywords = {Goniothalamus umbrosus,cancer,apoptosis,MCF-7,CEM-ss,HT29},
    abstract = {This current study, aims to investigate the ethyl acetate and methanol extracts of <I>Goniothalamus umbrosus</I> for their anticancer effects on several human cancer cells namely, the MCF-7 breast cancer, HT-29 colon cancer and CEM-ss leukemia cell lines using a 3 days MTT (3-(4 and 5-dimethylthiazol-2-yl)-2 and 5-diphenyltetrazolium bromide) assay. Morphological changes and probable mode of cancer cell death induced by bioactive <I>G. umbrosus</I> extract were examined. DNA laddering assay was performed to assess endo-nucleosomal fragmentation. The MTT assay results revealed that only the ethyl acetate extract has anticancer effects on human breast cancer cells (MCF-7). Half maximal Inhibitory Concentration (IC<SUB>50</SUB>) of the ethyl acetate extract was found to be 24.5&plusmn;0.12 &#956;g mL<SUP>-1</SUP>. Both inverted and fluorescence microscopic studies demonstrated that treated MCF-7 breast cancer cells using IC<SUB>50</SUB> of the extract displayed a number of typical morphological changes. Appearance of membrane blebs, DNA condensation and fragmentation are significant signs of apoptosis, were observed. The above findings suggested that the ethyl acetate extract of <I>Goniothalamus umbrosus</I> has potential therapeutic effect towards human breast cancer cells that requires further investigations in future.}
    }