@article{MAKHILLRJBS200831210786,
    title = {Complementary DNA Cloning and Immunologic Characterization of a New<I> Platanus orientalis </I>Pollen Allergen, Pla or 1.0101},
    journal = {Research Journal of Biological Sciences},
    volume = {3},
    number = {12},
    pages = {1419-1425},
    year = {2008},
    issn = {1815-8846},
    doi = {rjbsci.2008.1419.1425},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2008.1419.1425},
    author = {Nazanin Pazouki,Mojtaba Sankian,Taher Nejadsattari,Ramezan-Ali Khavari-Nejad and},
    keywords = {Allergen characterization,cDNA cloning,cyclophilin,invertase inhibitors,MALDI-TOF-MS,Platanus orientalis,recombinant allergen,SDS -PAGE immunoblotting},
    abstract = {Oriental Plane trees, as <I>Platanus orientalis</I>, are an important source of airborne allergens in cities of the southwest Asia and southeast Europe. Diagnosis of type I allergy essentially depends on the availability of defined allergens, which can be provided by recombinant technology. This study was aimed to investigate molecular properties of a <I>Platanus orientalis </I>pollen allergen, designated as Pla or 1.0101 (accession number EU296476), to produce its immunoreactive recombinant counterpart in <I>Escherichia coli</I>. Molecular characterization of the <I>Platanus orientalis </I>pollen allergen was performed using, cDNA cloning, expression of the recombinant allergen in <I>Escherichia coli</I> and IgE immunoblotting of recombinant allergen<I>. </I>The 18 kDa allergen (Pla or 1.0101) was identified as an important IgE-binding component of <I>Platanus orientalis </I>pollen. The  Pla  or  1.0101-specific cDNA  sequences  were  amplified,  using  specific  primers  based  on the N and C-terminal sequence of a Pla or 1.0101 homologue in <I>Platanus acerifolia</I>, Pla a 1. Sequencing corresponding Pla or 1.0101 cDNA revealed an open reading frame of 513 bp coding for 170 amino acid residues. The recombinant Pla or 1.0101 was produced by pET102/D-TOPO <I>E. coli</I> expression system. IgE-binding to the recombinant form of Pla or 1.0101 was proven by immunoblot and specific ELISA.}
    }