@article{MAKHILLRJBS200831110767,
    title = {Development of a PCR Primer and a Marker Band for Detection of <I>E. coli</I> from Various Sources Based on Arbitrary Primer Set},
    journal = {Research Journal of Biological Sciences},
    volume = {3},
    number = {11},
    pages = {1327-1332},
    year = {2008},
    issn = {1815-8846},
    doi = {rjbsci.2008.1327.1332},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2008.1327.1332},
    author = {Nagi A. AL-Haj,Mariana N. Shamsudin,Raha A. Rahim and},
    keywords = {Random Amplified Polymorphic DNA (RAPD),uropathogenic E. coli (UPEC),Neonatal Meningitis-associated E. coli (MNEC),University Putra Malaysia (UPM),primer,sequence},
    abstract = {Although, PCR methods aimed on the detection of genes associated with the pathogenicity of <I>Escherichia coli</I> have been reported, tests allowing the direct identification of this serotype are rare. In this study the Random Amplified Polymorphic DNA (RAPD) fingerprinting technique allowed genetic diversity assessment of 25 <I>E. coli</I> isolates of various sources. A highly significant finding from the DNA fingerprinting is the display of a predominant band at a size of 308 bp when arbitrary OPAE-10 primer was used. After sequencing this fragment primer called <I>secD</I> was designed to be used as PCR primer. <I>secD</I> primer pairs was highly specific to detect all isolates including <I>E. coli</I> O157: H7.}
    }