@article{MAKHILLRJBS20083910708,
    title = {Comparison of Techniques to Determine the Ruminal and Post-Ruminal Protein Disappearance of Various Oilseed Meals},
    journal = {Research Journal of Biological Sciences},
    volume = {3},
    number = {9},
    pages = {1028-1033},
    year = {2008},
    issn = {1815-8846},
    doi = {rjbsci.2008.1028.1033},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2008.1028.1033},
    author = {M. Danesh Mesgaran,H. Jahani-Azizabadi and},
    keywords = {Protein disappearance,3-step procedure,in vitro enzymatic procedure,oilseed meal},
    abstract = {Ruminal, post-ruminal and total tract crude protein disappearance of cottonseed meal containing 105 (CSMH) or 65 (CSML) g oil per kg of dry matter (DM), soybean meal (SBM), rapeseed meal (RSM) and sunflower meal (SFM), originating from Iranian plant varieties, were measured using <I>in situ</I> mobile bag, three-step <I>in situ</I>/<I>in vitro</I> (3-step) and <I>in vitro</I> enzymatic procedures. For the <I>in situ</I> mobile bag technique, 4 Holstein steers (450±50 kg, body weight) fitted with ruminal fistulae and T-shaped intestinal cannulae were used. Three-step procedure was followed by rumen incubation of samples for 12 h and enzymatic incubation of ruminal undegradable samples. <I>In vitro</I> enzymatic technique was followed by 1 h borate- phosphate buffer incubation, then 4 h in protease solution as ruminal disappearance and enzymatic (pepsin and pancreatin) incubation of ruminal undegradable samples. A significant (p&lt;0.01) effect was found between the procedures to measure ruminal, post-ruminal and total tract CP disappearance. The protein disappearance of oilseed meals in the rumen and post-rumen determined <I>in vitro</I> enzymatic procedure tended to be higher than 3-step technique. Post-ruminal protein disappearance measured with <I>in vitro</I> procedure was higher (p&lt;0.01) than <I>in situ</I> mobile bag and 3-step techniques (<I>In vitro</I> =  0.75, <I>In situ</I> mobile bag = 0.74 and 3-step = 0.60; S.E.M = 0.023, respectively). There was a significant (p&lt;0.01) effect of feeds on ruminal, post-ruminal and total tract CP disappearance. The CSMH<SUB> </SUB>had a lower (p&lt;0.01) ruminal CP disappearance, while the SFM had higher value (0.58 and 0.91, respectively). The CSMH had lower ruminal CP disappearance compared with CSML (0.58 vs. 0.68, respectively). The CSML<SUB> </SUB>had a lower (p&lt;0.01) post-ruminal CP disappearance, while the SBM had higher value (0.59 and 0.81, respectively). In addition, our results indicated that when used to assess post-ruminal disappearance of oilseed meal protein, the 3-step method can underestimate the disappearance of protein.}
    }