@article{MAKHILLRJBS20072710471,
    title = {Delipidation of a Pestivirus: Viral Inactivation and Vaccine Development in Large Animals},
    journal = {Research Journal of Biological Sciences},
    volume = {2},
    number = {7},
    pages = {706-712},
    year = {2007},
    issn = {1815-8846},
    doi = {rjbsci.2007.706.712},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2007.706.712},
    author = {Bill E. Cham,A.D. Shannon,X. Gu and},
    keywords = {Delipidation,virus inactivation,vaccine development,BVDV,lipid-associated virus},
    abstract = {Lipid Associated Viruses (LAVs) including HIV, hepatitis B, C and SARS are amongst the most malevolent viruses. These and a variety of other viruses have an outer lipid envelope which maintains inserted viral peptides in the &quot;correct&quot; functional conformation and orientation. Most solvents disrupt the lipid envelope and destroy infectivity but these solvents also result in a loss of antigenicity. Specific organic solvents consisting of butanol and Diisopropyl Ether (DIPE) delipidate the whole virion rendering it non-infective, but antigenic. In large animals such as cattle, delipidation of large amounts of the pestivirus Bovine Viral Diarrhea Virus (BVDV) result in the inactivation of the virus shown by <I>in vitro</I> and <I>in vivo</I> testing. The inactivated BVDV preparation when used as a vaccine results in anti-E2 antibody production in all vaccinated animals. Delipidation of lipid-associated enveloped viruses with specific organic solvents has potential as the basis for development of vaccines.}
    }