@article{MAKHILLRJBS20072410427,
    title = {Detection and Identification of <I>Cryptosporidium</I> species in Water Samples from a River in Ardabil City, Northwestern Iran},
    journal = {Research Journal of Biological Sciences},
    volume = {2},
    number = {4},
    pages = {498-502},
    year = {2007},
    issn = {1815-8846},
    doi = {rjbsci.2007.498.502},
    url = {https://makhillpublications.co/view-article.php?issn=1815-8846&doi=rjbsci.2007.498.502},
    author = {Behnam Mohammadi Ghalebin,Esmaeel Fallah,Mohammad Asgharzadh,Abdol Hassan Kazemi and},
    keywords = {Cryptosporidium species,PCR-RFLP,water,detection,indentification,Iran},
    abstract = {<I>Cryptosporidium</I> is an opportunistic parasite typically associated with large waterborne outbreaks. Surface waters contaminated with human and animal feces serve as main source for epidemic spread of <I>Cryptosporidium </I>parasites. In this study, we used a small-subunit rRNA-based PCR-Restriction Fragment Length Polymorphism (RFLP) technique to determine the prevalence and to characterize human-infective species of <I>Cryptosporidium</I> parasites in water samples collected from a stream in Ardabil city in Iran. Among 30 samples examined, 11 samples showed positive results. Restriction pattern analysis showed <I>C. andersony</I> as the most common species with 7 cases; followed by <I>C. parvum</I>, bovine genotype, with 3 cases and <I>C. suis</I> with 1 case. The results indicated that PCR-RFLP technique provides an applicable and feasible method for detection and identification of <I>Cryptosporidium </I>oocysts in environmental water samples. The results, furthermore, demonstrated that wildlife is the major source of <I>Cryptosporidium </I>oocysts in surface water resources in the study region.}
    }