@article{MAKHILLJAVA20222114676,
    title = {Polymorphism of Beta-Lactoglobulin and Kappa Casein Genes in Tunisian Native Goats},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {21},
    number = {1},
    pages = {25-33},
    year = {2022},
    issn = {1680-5593},
    doi = {javaa.2022.25.33},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2022.25.33},
    author = {Zahran,Zaabouti,Mounir and},
    keywords = {Local goat,β-lactoglobulin,kappa casein,PCR-RFLP,genotype},
    abstract = {This study investigated genetic polymorphisms in
the local goat populations of Tunisia. The polymorphisms of
the β-lactoglobulin (β-LG) and kappa casein (CNS3) genes
of two goat subpopulations in southern Tunisia (Jerid and
Nefzawa regions), known as Arbi and Serti, were assessed at
the DNA level. The DNA of 151 individual goats for β-LG
and 171 individuals for CNS3 were extracted and then
genotyped for β-LG using restriction fragment length polymorphism; SmaI restriction enzyme technique; and
HaeIII restriction enzyme technique for CNS3. CNS3 gene
digestion revealed two fragments of different sizes, 416 bp
and 229 bp, indicating the presence of the AA and AB
genotypes of kappa casein in the population. The AB
genotype (645/416/229 bp) was also detected, while the BB
genotype was completely absent in the oasis goat population.
The results of the β-LG gene revealed three different
genotypes, each characterized by a different number and size
of fragments. The homozygous form CC presented three
fragments of different sizes, 472, 181 and 50 bp, while the
other form TT presented only two fragments, 472 and 231
bp. Finally, the heterozygous form CT controlled four
fragments of different sizes, 472, 231, 181 and 50 bp. Allele
A of kappa-casein occurred at a higher frequency than allele
B in both the Arbi and Serti subpopulations and the overall
population. The β-lactoglobulin C allele was more common
than the T allele in the Arbi and Serti subpopulations and
throughout the population. The genotypic frequencies of
homozygotes were found to be higher than those of
heterozygotes in the two subgroups and within the total
population. At the CNS3 locus, the total population and the
two subpopulations (Arbi and Serti) were in Hardy-
Weinberg equilibrium, while deviation from Hardy-
Weinberg equilibrium was recorded at the β-LG locus.}
    }