@article{MAKHILLJAVA20212094671,
    title = {Technical Notes: Heparin Sodium Extraction from Broiler Intestines Based on Acid/Base
Methodology},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {20},
    number = {9},
    pages = {185-188},
    year = {2021},
    issn = {1680-5593},
    doi = {javaa.2021.185.188},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2021.185.188},
    author = {Tzen-,Yu-,Kuo-,I-,Ming- and},
    keywords = {Heparin sodium,broiler intestines,acid/base extraction,purification},
    abstract = {Heparin sodium is the raw material of heparin,
a blood coagulant commonly used in medical treatment.
Heparin is clinically used to prevent blood vessel
thrombosis. In the past, heparin sodium was mainly
extracted from porcine intestines. Here, an acid/base
methodology was modified and used to extract heparin
sodium from broiler viscera. Trypsin was added to
smashed chicken’s intestines at pH 8.5. D208 resin was
then applied to adsorb heparin, followed by a process
through a series of acid/base changes. Crude heparin
sodium product, recovered by drying, was purified with
permanganate oxidation followed by centrifuging. Final
heparin products were harvested from the precipitation.
From 0.2 kg raw materials of a mixture of chicken’s
intestines, 23.80 g crude heparin was obtained, while
31.40 mg heparin was isolated and recovered after
purification. Accordingly, 119.00 g crude heparin and
159.00 mg purified heparin can be harvested from 1 kg
raw materials. The yield of heparin isolated from broiler
intestines approximates that from porcine. This study
indicates that chicken can serve a new source of
pharmaceutical heparin, especially given an outbreak of
African swine fever in Asia.}
    }