@article{MAKHILLJAVA20181734556,
    title = {Transcriptome Analysis Identified the Regulation of Dietary Zinc on
Hepatic Lipid Metabolism in Rats},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {17},
    number = {3},
    pages = {61-65},
    year = {2018},
    issn = {1680-5593},
    doi = {javaa.2018.61.65},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2018.61.65},
    author = {Jianfeng,Jianyi,Xiaoyan and},
    keywords = {cDNA microarry,gene expression,liver,Zinc,fatty acid metabolism,desaturation},
    abstract = {The objective of the study was to investigate the regulation of dietary Zinc (Zn) on transcription of
key enzymes involved into hepatic lipid metabolism. Rats were fed a Zinc-Adequate diet (ZA, 46.39 mg Zn/kg
basis diet), a Zinc-Deficient diet (ZD, 3.20 mg Zn/kg basis diet) and a Zinc-Overdose diet (ZO, 234.39 mg Zn/kg
basis diet) for 5 weeks. Pair-Fed group (PF) rats were fed zinc-adequate diet with food intake of ZD group. Zinc
concentrations of liver and femur and activity of Alkaline Phosphatase (ALP) in ZD group were significantly
decreased compared with PF group whereas those in ZO group were markedly increased compared with ZA
group. Content of oleic acid was markedly decreased in ZD but the contents of palmitic acid and oleic acid were
significantly increased in ZO. Concentrations of hepatic Free Fatty Acid (FFA) and Triglyceride (TG) were
markedly raised in ZD group whereas these in ZO group was reduced. Of 9753 genes analyzed by cDNA
microarray, 13 genes related to hepatic lipid metabolism showed altered expression levels in livers of ZD and
ZO rats, respectively with. The changes of four genes were confirmed by relative quantitative real-time RT-PCR.
Compared with PF group, mRNA level of Acetyl-CoA Carboxylase (ACC) was significantly increased but
Stearoyl-CoA Desaturase-1 (SCD-1) was reduced in ZD group. Relative to ZA group, mRNA levels of Fatty
Acid Synthase (FAS) and ACC was obviously decreased in ZO group but these of Carnitine Palmiroyl
Transferase-1 (CPT-1) and SCD-1 were significantly elevated. These results indicated that dietary zinc might
affect hepatic fatty acid synthesis, β-oxidation and desaturation through regulation on FAS, ACC, CPT-1 and
SCD-1 at the transcriptional level.}
    }