@article{MAKHILLJAVA201514104506,
    title = {Biofilm-Producing <I>Staphylococcus aureus</I> Screening in Poultry Farms and Abattoirs},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {14},
    number = {10},
    pages = {308-314},
    year = {2015},
    issn = {1680-5593},
    doi = {javaa.2015.308.314},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2015.308.314},
    author = {A.M. and},
    keywords = {S. aureus,poultry,biofilm,abattoirs,PCR,sequence,MTP,CRA},
    abstract = {Surveillance on<I> Staphylococcus aureus</I> in poultry farms and poultry abattoirs in 2015 in Egypt was applied for determining the biofilm formation, an important virulence and antibiotic resistance determinant. Sixty-seven (68.3%) out of 98 poultry and environmental samples collected in this study were positive for <I>S. aureus</I>. For phenotypic analysis of biofilm formation, Microtitre Plate (MTP) and Congo Red Agar (CRA) tests revealed 63 (94.02%) and 59 (88.05%) positive samples, respectively. A high correlation (94.02%) between MTP and CRA tests was encountered. The PCR showed variable results for genes encoding Microbial Surface Components Recognizing Adhesive Matrix Molecules (MSCRAMMs) and for those encoding Polysaccharide Intercellular Adhesion/Poly-N-Succinyl-&#946;-1-6-Glucosamine (PIA/PNSG). Partial DNA sequencing of 606 nucleotides of the <I>icaR-icaA</I> fragment in 21 selected strains showed 98.2%-100% identity with two clearly distinct phylogroups. This study indicated that biofilm producing <I>S. aureus</I> are widely distributed in poultry and poultry abattoirs in Egypt.}
    }