@article{MAKHILLJAVA20151454475,
    title = {Expression of Recombinant Baculovirus Vetor Double-expressing HA1 Gene from H5 Subtype Avian Influenza Virus and Mature Chicken Interleukin-18 Gene},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {14},
    number = {5},
    pages = {136-139},
    year = {2015},
    issn = {1680-5593},
    doi = {javaa.2015.136.139},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2015.136.139},
    author = {Na},
    keywords = {Avian influenza virus,HA1 gene,mature ChIL-18 gene,eukaryotic expression,IL18 protein},
    abstract = {The Hemagglutinin (<I>HA1</I>) gene from H5 subtype Avian Influenza Virus (AIV) and mature Chicken Interleukin-18 (<I>mChIL-18</I>) gene were amplified by designing two pairs of primers according to the sequence published on GenBank. The <I>HA1</I> gene and <I>mChIL-18</I> gene were inserted into the downstreams of PH promoter and P10 promoter of baculovirus expression plasmid pFastBac<SUP>TM</SUP>Dual, respectively. The recombinant pFastBac<SUP>TM</SUP>dual-mChIL-18-HA1 (rIL18-HA1) was ransformed into DH10Bac competent cells and expressed in sf9 cells by IFA diction. The results showed that the recombinant protein IL18-HA1 (pIL18-HA1) was simultaneouly expressed in the same sf9 cells with IL18 protein and HA1 protein, respectively. It is the foundation for developing a neotype vaccine against AIV.}
    }