@article{MAKHILLJAVA201413154411,
    title = {Determination of Minimum Bactericidal Concentrations of Antimicrobials Used 
  for Canine Pyoderma Against <I>Staphylococcus pseudintermedius},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {13},
    number = {15},
    pages = {958-962},
    year = {2014},
    issn = {1680-5593},
    doi = {javaa.2014.958.962},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2014.958.962},
    author = {Kazuki,Aiko and},
    keywords = {Staphylococcus pseudintermedius,minimum bactericidal concentration,minimum inhibitory concentration,canine pyoderma,organism},
    abstract = {Minimum Bactericidal Concentration (MBC) is an important <I>in 
  vitro</I> parameter of antimicrobial potency but has not yet been measured for 
  <I>Staphylococcus pseudintermedius</I>, the main causative organism of canine 
  pyoderma. This study was carried out to determine the MBC of representative 
  antimicrobials against mecA-negative and -positive <I>S. pseudintermdius</I>. 
  Fifty nine mecA-negative and 33 mecA-positive isolates were tested for Minimum 
  Inhibitory Concentration (MIC) of eight antimicrobials by Broth Microdilution 
  Method. Subsequently, MBC was determined as concentration at which &ge;99.9% 
  decrease in bacterial counts was achieved by sub-cultivating suspensions at 
  concentrations greater than MIC. As a result, cephalexin (LEX), Amoxicillin-Clavulanic 
  acid (AMC), Orbifloxacin (ORB) and Fosfomycin (FOF) had MBC:MIC ratio of &le;4 
  whereas trimethoprim-Sulfamethoxazole (SXT) had MBC:MIC ratio of &le;16. In 
  contrast, Minocycline (MIN), Erythromycin (ERY) and Clindamycin (CLI) had MBC:MIC 
  ratio of &ge;4. In mecA-negative isolates, among the tested antimicrobials, 
  FOF had the lowest MBC-resistance rate (8.5%) followed by AMC (13.6%) LEX (16.9%) 
  ORB (33.9%) SXT (33.9%) whereas MIN, ERY and CLI had high MBC-resistance rates 
  (71.2-96.6%). On the other hand, in mecA-positive isolates, all tested antimicrobials 
  had &gt;50% of MBC-resistance rates (69.7-100%). Notably, the resistant-level 
  MIC value of MIN was not detected in either mecA-negative or -positive isolates. 
  The present data indicate that FOF is a superior bactericidal drug against mecA-negative 
  isolates whereas MINO can be effective bacteriostatic drugs for mecA-positive 
  and -negative isolates of <I>S. pseudintermedius</I>.}
    }