@article{MAKHILLJAVA20141324180,
    title = {Effect of Dietary Vitamin E Supplementation on Oxidative Stress Response of Acute Nitrite Exposure in Sea Cucumber (<I>Apostichopus japonicus </I>Selenka) Juveniles},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {13},
    number = {2},
    pages = {77-88},
    year = {2014},
    issn = {1680-5593},
    doi = {javaa.2014.77.88},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2014.77.88},
    author = {Lei,Zuoyong,Baojie,Mei and},
    keywords = {Sea cucumber (Apostichopus japonicus Selenka),vitamin E,nitrite stress,antioxidant enzyme,Reactive Oxygen Species (ROS),Response Surface Methodology (RSM)},
    abstract = {Different amounts of vitamin E supplementation were added 
  to feed sea cucumber (<I>Apostichopus japonicus</I> Selenka) juveniles (2.5&plusmn;0.15 
  g) testing to reduce nitrite stress. Commercial feed was used as the control 
  diet, 3 experimental diets containing vitamin E supplementation (150, 250 and 
  350 mg kg<SUP>-1</SUP> diet), respectively was designed to experiment for 45 
  days. The specimens were exposed to three different concentrations (0.5, 1.0 
  and 1.5 mg L<SUP>-1</SUP>) of nitrite for 4, 8 and 12 h at four different time 
  points (0, 15, 30 and 45 days). Hydroxyl free radical (-OH), Malondialdehyde 
  (MDA), Total Antioxidant Capacity (T-AOC), Superoxide Dismutase (SOD) and Catalase 
  (CAT) were measured. The control group&#146; 
  level of -OH and MDA turned higher whereas T-AOC turned lower and SOD and CAT 
  activity elevated after exposed to nitrite solution. The experimental group&#146; 
  level of -OH and MDA turned lower whereas T-AOC turned higher. The results suggested 
  vitamin E supplementation diets could reduce nitrite stress response and increase 
  the antioxidant capacity. The optimal amount of vitamin E supplementation ranged 
  from 180-260 mg kg<SUP>-1</SUP> and number of feeding days ranged from 33-35 
  days were obtained according regress equations.}
    }