@article{MAKHILLJAVA201413124357,
    title = {Effects of Nelin Gene Expression on Phenotypic Switch of Vascular Smooth Muscle Cells},
    journal = {Journal of Animal and Veterinary Advances},
    volume = {13},
    number = {12},
    pages = {706-710},
    year = {2014},
    issn = {1680-5593},
    doi = {javaa.2014.706.710},
    url = {https://makhillpublications.co/view-article.php?issn=1680-5593&doi=javaa.2014.706.710},
    author = {Shuguang,Changan,Shiyong and},
    keywords = {Nelin,vascular smooth muscle cells,phenotypic switch,cytoskeleton,acetylcholine},
    abstract = {To observe the effects of Nelin gene expression on phenotypic 
  switch of Vascular Smooth Muscle Cells (VSMC). VSMC were induced from synthetic 
  phenotype to contractile phenotype by serum starvation and Nelin mRNA expression 
  levels of different phenotypes of VSMC were checked by using RT-PCR. siRNA was 
  then used to silence Nelin, coomassie blue staining and Acetylcholine (Ach) 
  stimulation were used to observe the changes of VSMC cytoskeleton and contractile 
  functions. Nelin mRNA expression in serum stimulation and serum starvation at 
  24, 48 and 72 h group was 0.1940+0.0971, 0.7114+0.1265, 0.7270+0.1026 and 0.7841+0.1240. 
  The difference was statistically significant (p<0.05). In serum starvation 
  process, VSMC cytoskeleton was reconstructed into uniform, dense bundles from 
  sparse fuzzy networks and regained contractile function. Serum starvation-induced 
  cytoskeletal remodeling was blocked and Ach-induced cell contraction disappeared 
  when Nelin expression was suppressed.}
    }